Description
Our specialty Liquid Culture Mix containing Corn Dextrose and Light Malt Extract (DLME) is ideal for large-scale expansion and growth of mushroom mycelium. This formulation provides far superior nutrient levels over honey, Karo Syrup, or glucose syrup. This mix is best used for extremely vigorous strains like Reishi, Oysters or Swordbelt.
We also sell another product – Using our Supercharge Liquid Culture Pre Mix with any of these vigorous species, like Reishi, will produce an overabundance of strong growth, which is hard to pull into the syringe with a regular needle. That’s why this recipe, which is not as nutrient-dense, is great.You should only use DLME recipe over our Supercharge LC Pre-mix, if you have issues with excessive growth that is hard to pull into the needle.
The difference in both the recipe is the Presence of Peptone.
Key Ingredients and Their Benefits
Light Malt Extract (DLME)
- Derived from barley
- Accelerates colonization in grain spawn and agar.
Corn Dextrose
- Provides readily available simple sugars
- Contributes essential minerals and vitamins
- Ensures a clear culture medium for easy contamination detection
Preparation Guidelines
Mixing ratio:
-2% solution: 10g mix per 500ml water (This is a standard Mix)
-4% solution: 20g mix per 500ml water (More the sugar more energy but mix above 4% is toxic to mycelium)
-100gm can make up to 2.5%-5 Litres of Liquid cultures
-250gm can make up to 6.25%-12.5 Litres of Liquid cultures
Sterilization:
-Autoclave at 15 PSI for 45 minutes
-Alternative microwave method available (contact for details)
Cooling and inoculation:
-Allow to reach room temperature before adding culture.
Incubation:
-Store in darkness for 1-2 weeks
-Stir regularly to break up mycelium.
Our new recipe is 6 times more clearer than our previous mix without any sedimentation.
Storage:
Keep the mix away from any moisture, store in an air tight container.
