Description
Indole-3-Butyric Acid (IBA) Powder 5g is a plant growth regulator extensively utilized to enhance root growth in plant tissue culture processes.
Advantages
- Simple and convenient to use.
- Commonly added to tissue culture media, such as MS media.
- Promotes the development of adventitious roots and callus formation.
- Often combined with other plant hormones to achieve optimal growth and development.
Instructions for Using Indole-3-Butyric Acid (IBA) Powder
Follow these steps to incorporate PCT’s IBA powder into your tissue culture media preparation:
- Prepare the liquid tissue culture medium using PCT’s MS, DKW, or other suitable media, depending on your plant’s requirements.
- Add sugar proportional to the volume of media being prepared (typically, 30 grams of sugar per liter), 1 ml/L PPM™, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar.
- Incorporate the desired amount of IBA from the pre-made IBA stock solution.
- Sterilize the medium by autoclaving it for 20 minutes at 121℃ and 15 psi.
Your tissue culture medium is now ready for use!
For additional details about Indole-3-Butyric Acid (IBA).
Also see Plant Cell Blog.
