Description
Make your own MYAG agar petri dish contamination-free.
General MYAG with antibacterial properties
This is a premium product revolutionizing mushroom cultivation.
MYAG is a gelatinous medium that is used to purify, germinate, isolate and manipulate mushroom mycelium to reduce the chances of contamination drastically due to its antibacterial compound.
Why use MYAG instead of just MYA?
Most of the spores obtained, whether from wild, homegrown, or trustable spore sellers, Might include bacterial contamination. That is due to methods of spore collection, preservation, and transportation (Such as spore syringes and spore prints). In several cases, when spores are infected, they carry bacteria attached to them and introduce them to the Agar media. This will result in fast-growing bacterial spores that make it difficult for mushroom spores to germinate (as they are slower than bacterial spores).
On the other hand, when infected spores are introduced to MYGA (with antibacterial properties), the mushroom spores will germinate and grow, leaving behind bacterial contamination. That is because the cells composing the mycelium (Hyphae) have long and sharp filaments, which carry reduced populations (if any) of bacteria when they pass through the antibacterial properties. This antibacterial powder is an antibiotic commonly used to treat bacterial infections. In Agar mycelium cultures, it has been demonstrated to inhibit bacteria to a confinable level.
The uses of MYAG with antibacterial properties include
- Germinating spores from a spore syringe or spore print has a likelihood of contamination.
- Purify mycelium from other contaminated agar plates
- Clone wild mushroom tissue, which has a likelihood of contamination
Quantity specifications:
These quantities are measured based on 60*15mm size Petri dishes:
The number of plates made will vary according to pouring techniques; we recommend using just enough to cover the surface of the petri dish.
- 21 gm makes 35 Agar Plates (Use 500ml of hot water)
- 42 gm makes 70 Agar Plates (Use 1000ml of hot water)
- 63 gm makes 105 Agar Plates (Use 1500ml of hot water)
- 84 gm makes 140 Agar Plates (Use 2000ml of hot water)
- 168gm makes 280 Agar Plates (Use 4000ml of hot water)
Instructions
- Dissolve your MYAG packet in the specified ratio of hot water (i.e.1000, ml water for 42 gm of MYAG) and stir thoroughly with a spoon. If you have a magnetic stirrer, this will mix the solution properly. The content needs to be poured slowly and not all at once.
- Autoclave or pressure cook for 15 minutes at 15 psi.
- Pour into Petri dishes when it reaches 50 degrees Celsius (Approximately hot enough to hold the flask/ jar with one hand), and let it set for 10 minutes. Do not stir the solution after taking it out of Autoclave, as this would trap air bubbles in this solution.
- Once the Petri dishes are set, inoculate them and wrap them with parafilm. Keeping the plates aside for a week is recommended to check for contamination.
- Petri dishes are best to be stored upside down to avoid condensation.
Do not have a pressure cooker? No problem; message us, and we will send you detailed instructions on how to use a microwave for sterilizing along with your purchase. Please check our listing, Make Agar Plates and see what you need
First, you would need a Reagent Bottle If made in a Microwave, a 500ml reagent bottle would fit. If using a Sterilizer, then a 1000ml reagent bottle should fit.
We have relied on this Mix to experiment with different varieties in our lab.
Check out our other listings to add Liquid Cultures and see more Pre Mixes. We also sell other types of Mushroom-Growing Equipment.
You can also buy antibacterial powder without yeast from our store.
