Plant Cell Tissue Culture Starter Kit

Description

Curious about Tissue Culture but unsure how to begin?
The PCT Starter Kit is the perfect way to dive into tissue culture and develop your skills!

What’s Included in the Kit?

• MS Media with Vitamins: Prepare up to 5 liters of tissue culture media.
• 100 grams of Supreme Tissue-Culture-Grade Agar: Provides essential support for plant growth.
• 10 ml of PPM™: Protects your cultures from contamination.
• 2 Square Culture Vessels: Creates an ideal environment for plant growth and development.
• 1 Disposable Syringe: Simplifies precise ingredient measurement for perfect tissue culture recipes.

Product Information

Square Tissue Culture Vessels with Lids

• Description: Heat-resistant and autoclavable.
• Features: Comes with lids.
• Capacity: Growth area of 6.15 square inches.
• Shape: Square design.
• Color: Transparent.
• Dimensions: 3″ x 3″ x 4″.

Supreme Tissue-Culture-Grade Agar

• Description: A polysaccharide derived from the red alga Rhodophyceae, processed via bleaching and hot water extraction. Recommended usage rate: 6–12 g/L. Supreme agar ensures excellent clarity for culture media.
• Gel Strength: 1050 GM/CM².
• Packaging Options: 100g, 500g, 1kg in poly bottles.
• Applications: Ideal for solid microbial culturing media, it’s used at concentrations of 0.5–1.0% (w/v) to solidify plant tissue culture media. Essential for in vitro root development.

Murashige & Skoog (MS) Medium

• Description: MS medium with vitamins and calcium chloride (without sucrose and agar).
• Technical Details: Supports plant growth and shoot proliferation across both monocot and dicot tissue cultures.
• Formula Weight: 4.54 g/L.

Preparation Instructions:

• 1. Dissolve 4.54 g of powder in 600 ml of distilled or deionized water at room temperature (15–30°C).
  2. Rinse the media vial with a small amount of distilled water to remove residue.
  3. Add desired heat-stable supplements before autoclaving.
  4. Stir until dissolved. If needed, lower pH to ~3.0 for better dissolution.
  5. Adjust pH between 5.4 and 5.8 using 1N HCl/NaOH/KOH.
  6. Top up to 1 liter with distilled water. Mix gently and heat until clear. Avoid boiling or reheating.
  7. Autoclave at 121°C (15 psi) for 15 minutes.
  8. After cooling to 45–50°C, add heat-sensitive supplements aseptically.
• Storage: Keep at 2–8°C, away from moisture and direct light. Use before the expiry date. Opened containers should be used immediately or stored in desiccators at 2–8°C.

Plant Preservative Mixture (PPM™)

• Description: PPM™ is a heat-stable preservative and biocide designed to prevent or minimize microbial contamination in plant tissue culture.
• Key Benefits:
  • Effectively inhibits bacteria and fungi spore germination.
  • Maintains in vitro seed germination, callus proliferation, and regeneration.
  • Heat-stable and autoclavable with culture media.
  • More cost-effective than traditional antibiotics.
  • Reduces contamination from airborne, waterborne, and human contact sources.
  • Can be used to mitigate endogenous contamination in some cases.
• Usage: PPM™ is ideal as a standard ingredient in tissue culture media and protects against contamination even with rigorous sterile practices.

Yo can find more information in Plant Cell Technology

Also see Plant Cell Blog.